Monday, October 27, 2008
Monday, October 20, 2008
Friday, October 17, 2008
I work in a molecular lab, and this is what i do:
My boss, Dean Glawe-a plant pathologist and mycologist-picks out what powdery mildew samples he wants me to sequence. I am his lab technician.
Remove powdery mildew from plant leaves with a scalpel.
Break open the cells with a lysing matrix of garnet sand and ceramic balls.
Use Qiagen kit for DNA extraction.
PCR (Polymerase Chain Reaction) on extracted DNA with primers that amplify only (hopefully) the segment you are looking for.
Gel electrophoresis of PCR product to visually confirm correct amplification before investing more time in following procedures.
Exosap-IT procedure to clean the sample of non-target DNA.
Prepare samples of amplified DNA to be sent to Sequencing facility.
Three days later, we know the sequence of base pairs (A,T,C or G) and can compare our segment with others whether online or previously extracted in our lab.
Amazing!!! Science is amazing!!!
Sunday, October 12, 2008
The Puget Sound Mycological Society, PSMS, had their big mushroom show this weekend. It is a community awareness show/fundraiser for the club. I could not get the picture to be horizontal :(
The club members collect all the mushrooms and fungi they can find in the area, sort them by genus, and display them with name tags for the community to see.
There were mushroom books, posters, cooking classes, and on site identification of your own backyard mushrooms.
This really did smell like cherries!
The famous Amanita muscaria
The delicious Boletus edulus
Adam's favorite: Hericium, lion's mane, or bear's head.
It's edible, too!
My favorite: the Coral Fungi, Ramaria and Clavaria, etc.